Hangzhou Lane Technology Co.,Ltd
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Generic Name：Human trefoil factor(TFF1) ELISA Kit.
Purpose
This kit allows for the determination of TFF1 concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human TFF1 level in the sample，use Purified Human TFF1antibody to coat microtiter plate wells, make solid-phase antibody, then add TFF1 to wells, Combined TFF1 antibody which With HRP labeled goat anti-Human become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TFF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.